1KWK
Crystal structure of Thermus thermophilus A4 beta-galactosidase in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 97.592, 97.592, 128.898 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.620 - 2.200 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.20000 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.620 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | 0.325 |
| Number of reflections | 36622 | |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG8000, sodium acetate trihydrate, sodium cacodirate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
