1KWG
Crystal structure of Thermus thermophilus A4 beta-galactosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 97.737, 97.737, 129.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.730 - 1.600 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.18400 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.900 * |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.730 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.041 | 0.289 |
| Total number of observations | 2467779 * | |
| Number of reflections | 94451 * | |
| <I/σ(I)> | 16.2 | 6.04 |
| Completeness [%] | 100.0 * | 99.9 * |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 25 * | PEG 8000, sodium acetate trihydrate, sodium cacodyrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium acetate trihydrate | 0.2 (M) | |
| 2 | 1 | reservoir | PEG8000 | 20-25 (%(w/v)) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
