1KWA
HUMAN CASK/LIN-2 PDZ DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1996-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979, 1.033 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.800, 97.000, 92.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.930 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.29800 |
| Structure solution method | MAD, SIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.800 * |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.032 * | 0.293 * |
| Total number of observations | 50697 * | |
| Number of reflections | 14216 | |
| <I/σ(I)> | 21.6 | 3.1 |
| Completeness [%] | 88.4 | 78.1 |
| Redundancy | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 21 * | PROTEIN WAS CRYSTALLIZED IN 1.8M AMMONIUM SULFATE AND 100MM MES PH 6.5 AT 21 DEGREES C., temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | hCASK | 4 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.9 (M) | |
| 3 | 1 | drop | MES | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 5 | 1 | reservoir | MES | 100 (mM) |
