1KV1
p38 MAP Kinase in Complex with Inhibitor 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-01-05 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.000, 74.400, 77.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.500 |
R-factor | 0.221 * |
Rwork | 0.221 |
R-free | 0.28400 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.046 | |
Total number of observations | 31423 * | |
Number of reflections | 12621 | |
Completeness [%] | 94.0 | 60 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 | 21-25 * | Pav, S., (1997) Protein Sci., 6, 242. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 6.0 (mg/ml) | |
2 | 1 | drop | inhibitor | 1.4 (mM) | |
3 | 1 | reservoir | PEG1500 | 28-32 (%(w/v)) | |
4 | 1 | reservoir | dithiothreitol | 5 (mM) |