1KU1
Crystal Structure of the Sec7 Domain of Yeast GEA2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-03-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.948 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.052, 75.881, 76.199 |
Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
Resolution | 25.000 * - 1.930 |
R-factor | 0.214 * |
Rwork | 0.214 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 19.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | 1.970 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.080 | 0.326 |
Number of reflections | 52653 | |
<I/σ(I)> | 14.6 | 2.7 |
Completeness [%] | 97.0 | 94.4 |
Redundancy | 4.6 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | used microseeding * |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30-40 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | dithiothreitol | 1-4 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
6 | 1 | reservoir | sodium acetate | 0.1 (M) | or sodium citrate, pH5.0 |