1KTP

Crystal structure of c-phycocyanin of synechococcus vulcanus at 1.6 angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-16
Detector	ADSC QUANTUM 4
Spacegroup name	H 3 2
Unit cell lengths	186.346, 186.346, 59.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.212 *
Rwork	0.216
R-free	0.25400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i7y
RMSD bond length	0.005
RMSD bond angle	1.060 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.078 *	0.378 *
Total number of observations	419135 *
Number of reflections	51656 *
<I/σ(I)>	10	3
Completeness [%]	97.5 *	99.3 *
Redundancy	5.6	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	22 *	Adir, N., (2001) J. Mol. Biol., 313, 71. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	5 (%)
2	1	drop	Tris	50 (mM)
3	1	drop	protein	2.5-5 (mg/ml)