1KTN
Structural Genomics, Protein EC1535
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-01 |
| Detector | SBC-1 |
| Wavelength(s) | 0.9795, 0.9798, 0.9650 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.566, 53.558, 81.365 |
| Unit cell angles | 90.00, 109.97, 90.00 |
Refinement procedure
| Resolution | 38.650 - 1.400 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.078 | 0.309 |
| Number of reflections | 98786 | |
| <I/σ(I)> | 20 | 2.44 |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 6 | 4.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 298 | 20% PEG4K, 0.2M MgCl2, 0.1M Tris_HCl, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
