1KTL

The human non-classical major histocompatibility complex molecule HLA-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-07-12
Detector	RIGAKU RAXIS IV
Spacegroup name	P 31 2 1
Unit cell lengths	178.400, 178.400, 87.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 3.100
Rwork	0.238
R-free	0.28000
Structure solution method	RIGID-BODY REFINEMENT OF 1KPR
Starting model (for MR)	1kpr
RMSD bond length	0.027
RMSD bond angle	2.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.150
High resolution limit [Å]	3.100	3.100
Rmerge	0.059	0.418
Total number of observations	265243 *
Number of reflections	27568
<I/σ(I)>	19.4	2.1
Completeness [%]	94.2	84.1
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	22 *	ammonium sulfate, PEG 400, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP at 295K, pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.2 (M)
2	1	reservoir	PEG400	2 (%)
3	1	reservoir	Tris	100 (mM)	pH8.0