1KTG

Crystal Structure of a C. elegans Ap4A Hydrolase Binary Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.87
Spacegroup name	P 1 21 1
Unit cell lengths	57.563, 36.758, 68.941
Unit cell angles	90.00, 114.16, 90.00

Refinement procedure

Resolution	18.000 * - 1.800
R-factor	0.189 *
Rwork	0.188
R-free	0.22200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kt9
RMSD bond length	0.009 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.097 *	0.339 *
Total number of observations	62050 *
Number of reflections	24019
Completeness [%]	97.0 *	96.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *	293	PEG 4000, Magnesium chloride, Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	32 (%(w/v))
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium citrate	100 (mM)	pH5.6