1KRM

Crystal structure of bovine adenosine deaminase complexed with 6-hydroxyl-1,6-dihydropurine riboside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-12-12
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.54178
Spacegroup name	P 43 21 2
Unit cell lengths	80.030, 80.030, 141.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.500
R-factor	0.196
Rwork	0.196
R-free	0.24100 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.600 *
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.730
High resolution limit [Å]	2.500	2.500
Rmerge	0.050 *	0.187 *
Number of reflections	16108 *
Completeness [%]	99.2 *	100 *
Redundancy	8.9 *	7.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	293	Kinoshita, T., (1999) Acta Crystallogr.,Sect., D55, 2031. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.0-2.2 (M)
2	1	reservoir	PEG400	2 (%(v/v))
3	1	reservoir	HEPES	0.1 (M)	pH7.5
4	1	drop	protein	20 (mg/ml)
5	1	drop	Tris-HCl	50 (mM)	pH7.5
6	1	drop	purine riboside	10 (mM)