1KQX
Crystal structure of apo-CRBP from zebrafish
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9393 |
Spacegroup name | I 4 |
Unit cell lengths | 88.759, 88.759, 38.437 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.060 - 1.700 |
R-factor | 0.2032 |
Rwork | 0.195 |
R-free | 0.27600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | holo rat CRBPII |
RMSD bond length | 0.007 |
RMSD bond angle | 1.900 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 * | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.094 | 0.294 |
Total number of observations | 143455 * | |
Number of reflections | 16261 * | |
<I/σ(I)> | 4.6 | 2.1 |
Completeness [%] | 97.7 | 97.7 * |
Redundancy | 3.4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 4 * | PEG 4000, Ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
2 | 1 | reservoir | sodium citrate | 0.1 (M) | |
3 | 1 | reservoir | ammonium acetate | 0.2 (M) | pH5.6 |
4 | 1 | drop | protein | 8.0 (mg/ml) |