1KPU
High resolution crystal structure of the MHC class I complex H-2Kb/VSV8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-26 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 136.086, 88.121, 45.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.890 - 1.500 |
| R-factor | 0.18552 |
| Rwork | 0.184 |
| R-free | 0.20814 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vaa chains A and B |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.643 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 81282 | |
| <I/σ(I)> | 9.8 | 2.9 |
| Completeness [%] | 92.5 | 71.5 |
| Redundancy | 6.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | K/NA PHOSPHATE, MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
