1KPK
Crystal Structure of the ClC Chloride Channel from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.782 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.660, 152.525, 263.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.29 |
| Rwork | 0.290 |
| R-free | 0.30200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.075 | 0.505 |
| Number of reflections | 54294 | |
| <I/σ(I)> | 26.3 | 1.8 |
| Completeness [%] | 98.6 | 91.3 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 20 * | PEG 400, tris, sodium sulfate, lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | n-octyl-beta-maltoside | 45 (mM) | |
| 3 | 1 | drop | 75 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 5 | 1 | reservoir | PEG400 | 31-34 (%) | |
| 6 | 1 | reservoir | Tris | 50 (mM) | pH8.5 |
| 7 | 1 | reservoir | 50 (mM) | ||
| 8 | 1 | reservoir | 50 (mM) |
