1KON
CRYSTAL STRUCTURE OF E.COLI YEBC
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.96404,0.97927,0.97941,0.97945 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.560, 73.480, 39.260 |
Unit cell angles | 90.00, 97.89, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.200 |
R-factor | 0.273 |
Rwork | 0.273 |
R-free | 0.31700 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.331 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 11468 | |
Completeness [%] | 99.5 | 99 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Tris-Cl, postassium chloride, dithiothreitol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 22K |