1KNZ

Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	40.275, 85.359, 96.051
Unit cell angles	89.99, 90.00, 90.05

Refinement procedure

Resolution	22.000 - 2.450
R-factor	0.228 *
Rwork	0.228
R-free	0.28000
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000 *	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.045 *	0.149 *
Total number of observations	1328873 *
Number of reflections	46429
<I/σ(I)>	35	14
Completeness [%]	99.3	99.3
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	24 *	PEG 4000, ammonium sulfate, MES, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 4000
2	1	1	ammonium sulfate
3	1	1	MES

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%)
2	1	reservoir	MES/NaOH	50 (mM)	pH5.4
3	1	reservoir	ammonium sulfate	200 (mM)
4	1	drop	protein	5.5 (mg/ml)