1KNE

Chromo domain of HP1 complexed with histone H3 tail containing trimethyllysine 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-30
Detector	BRUKER SMART 6000
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	33.745, 76.943, 75.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.620 - 2.400
R-factor	0.246 *
Rwork	0.246
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	24.200 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.550
High resolution limit [Å]	2.400	2.400
Rmerge	0.083 *	0.297 *
Number of reflections	4086 *
<I/σ(I)>	47.2	15.1
Completeness [%]	99.5 *	95
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.1	10 *	Ammonium Sulfate, MES, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 283K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate
2	1	reservoir	MES		pH6.1