1KN1
Crystal structure of allophycocyanin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS X200B |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.300, 105.300, 189.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| Rwork | 0.193 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1ALL |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.900 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | |
| Number of reflections | 20096 | |
| <I/σ(I)> | 15.3 | 2.1 |
| Completeness [%] | 87.0 | 65 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | Liu, J.Y., (1998) Acta Crystallogr., D54, 662. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | 0.25 (M) | ||
| 3 | 1 | drop | ammonium sulfate | 0.4 (M) | |
| 4 | 1 | drop | HEPES | 50 (mM) | pH7.3 |
| 5 | 1 | reservoir | 0.4 (M) | ||
| 6 | 1 | reservoir | ammonium sulfate | 1.0 (M) |
