1KMQ
Crystal Structure of a Constitutively Activated RhoA Mutant (Q63L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9671 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.581, 73.340, 48.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 1.550 |
| R-factor | 0.16251 |
| Rwork | 0.161 |
| R-free | 0.19000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.036 * | 0.280 * |
| Total number of observations | 154149 * | |
| Number of reflections | 30966 | |
| <I/σ(I)> | 18.9 | 5.4 |
| Completeness [%] | 97.0 * | 85 * |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.9 | 277 * | PEG 8000, HEPES, dioxane, magnesium chloride, 2-mercaptoethanol, pH 6.9, VAPOR DIFFUSION, temperature 282K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 21 (%) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH6.9 |
| 4 | 1 | reservoir | dioxane | 20 (%) |
