1KMO

Crystal structure of the Outer Membrane Transporter FecA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-01
Detector	MARRESEARCH
Wavelength(s)	0.97800
Spacegroup name	P 21 21 2
Unit cell lengths	117.083, 88.086, 94.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.710 - 2.000
R-factor	0.207
Rwork	0.207
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	27.200 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.130
High resolution limit [Å]	2.000	2.000
Rmerge	0.090
Number of reflections	62452 *
<I/σ(I)>	11.1
Completeness [%]	92.3	0.71
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	284	Smith, B.S., (1998) Acta Cryst., D54, 697., Doublie, S., (1997) Methods Enzymol., 276, 532. *