1KLF
FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.077, 138.130, 215.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.800* |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.27900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qun |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.900 |
| High resolution limit [Å] | 2.800 * | 2.800 * |
| Rmerge | 0.069 | 0.478 |
| Total number of observations | 370427 * | |
| Number of reflections | 99135 | |
| <I/σ(I)> | 13 | 2.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 298 | ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.7 (mg/ml) | |
| 2 | 1 | drop | MES | 20 (mM) | pH6.5 |
| 3 | 1 | drop | alpha-D-mannose | 7 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.2 |
