1KK2
Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi-G235D mutant complexed with GDP-Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.870, 85.660, 57.330 |
| Unit cell angles | 90.00, 109.85, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.100 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| R-free | 0.23900 |
| Structure solution method | rigid body minimisation |
| Starting model (for MR) | 1kjz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 * | 0.304 * |
| Number of reflections | 27459 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 * | 297 | peg8000, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | MOPS | 10 (mM) | pH6.7 |
| 4 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 8-18 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
