1KJW

SH3-Guanylate Kinase Module from PSD-95

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	60.509, 60.509, 209.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.800
R-factor	0.23 *
Rwork	0.230
R-free	0.25000
Structure solution method	MAD
RMSD bond length	0.005 *
RMSD bond angle	1.080 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.077 *	0.351 *
Number of reflections	37032
Completeness [%]	99.4	94.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7	288	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	PEG400	2 (%)
4	1	reservoir	HEPES	10 (mM)	pH7.0