1KJV
TAP-B-associated rat MHC class I molecule
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-28 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.95007 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.397, 50.522, 200.073 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 * - 1.480 |
R-factor | 0.16404 |
Rwork | 0.162 |
R-free | 0.19100 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ed3 molecule 1 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.560 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.500 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.049 * | 0.693 * |
Number of reflections | 82429 | 2721 * |
<I/σ(I)> | 32.4 | 2 |
Completeness [%] | 99.3 | 100 |
Redundancy | 3.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 17 * | 26% PEG 4000, 0.2 M Li2SO4, 2% MPD, 0.1 M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 26 (%(w/v)) | |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | reservoir | MPD | 2 (%(v/v)) | |
5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |