1KJT
Crystal Structure of the GABA(A) Receptor Associated Protein, GABARAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9333 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.720, 56.040, 64.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.235 * |
| Rwork | 0.235 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.146 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 * | 0.439 * |
| Total number of observations | 56243 * | |
| Number of reflections | 7790 * | |
| Completeness [%] | 99.4 * | 95.5 * |
| Redundancy | 3.8 * | 3.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 * | 293 | PEG 2000MME, Tris HCL, nickel chloride, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH7.2 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | protein | 25 (mg/ml) | |
| 4 | 1 | reservoir | Tris | 0.1 (mM) | pH8.5 |
| 5 | 1 | reservoir | PEG2000MME | 30 (%(w/v)) | |
| 6 | 1 | reservoir | 5 (mM) |
