1KJM

TAP-A-associated rat MHC class I molecule

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-04
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 21 21 2
Unit cell lengths	110.049, 109.566, 45.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 2.350
R-factor	0.24935
Rwork	0.247
R-free	0.26800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ED3 MOLECULE 1
RMSD bond length	0.010 *
RMSD bond angle	1.520 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.800	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.080 *	0.423 *
Number of reflections	22313	2120 *
<I/σ(I)>	12.7	1.86
Completeness [%]	94.9	92
Redundancy	2.8 *	2.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	17 *	2M (NH4)2SO4, 1% glycerol, 0.1 M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	glycerol	1 (%(v/v))
4	1	reservoir	Tris-HCl	0.1 (M)	p8.5