1KIX

Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	F 4 3 2
Unit cell lengths	284.000, 284.000, 284.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.700
R-factor	0.208
Rwork	0.208
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alpha N-terminal domains from alpha-beta-ssDNA complex (1OTC)
RMSD bond length	0.007
RMSD bond angle	1.340 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.760
High resolution limit [Å]	2.700	2.700
Rmerge	0.075	0.548
Total number of observations	135022 *
Number of reflections	27195
<I/σ(I)>	16.1	2
Completeness [%]	98.3	94.5
Redundancy	5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Ammonium Sulfate, Xylitol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Ammonium Sulfate
2	1	1	Xylitol
3	1	1	HEPES

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop	oligonucleotide	20-50 (%)	molar excess
3	1	reservoir	ammonium sulfate	2.0-2.1 (M)
4	1	reservoir	xylitol	5 (%(w/v))
5	1	reservoir	HEPES	50 (mM)	pH7.5
6	1	reservoir	dithiothreitol	2 (mM)
7	1	reservoir	sodium azide	0.02 (%(w/v))