1KHH
Crystal Structure of Guanidinoacetate Methyltransferase from Rat Liver: A Template Structure of Protein Arginine Methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 54.250, 54.250, 156.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.28400 |
| Structure solution method | SIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.280 |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.047 | 0.139 |
| Total number of observations | 109295 * | |
| Number of reflections | 15472 * | |
| <I/σ(I)> | 11.4 | 5 |
| Completeness [%] | 98.0 | 83.4 |
| Redundancy | 6.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
| 2 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 3 | 1 | reservoir | SAH | 2 (mM) | |
| 4 | 1 | reservoir | GAA | 1 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 8 (%(w/v)) | |
| 6 | 1 | drop | protein | 10 (mg/ml) |
