1KH6
Crystal Structure of an RNA Tertiary Domain Essential to HCV IRES-mediated Translation Initiation.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9204, 0.9197, 0.9273, 0.9020 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 66.532, 66.532, 95.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.279 * |
| Rwork | 0.283 |
| R-free | 0.28300 * |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.220 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.800 | 2.800 * |
| Rmerge | 0.078 * | 0.536 * |
| Total number of observations | 72167 * | |
| Number of reflections | 9652 | |
| Completeness [%] | 98.3 * | 95.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 * | 303 | 30% MPD, 0.1 M sodium citrate pH 5.6, 0.2 M ammonium acetate, 2.5 mM magnesium chloride, and 1 mM spermidine, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | sodium citrate | ||
| 3 | 1 | 1 | ammonium acetate | ||
| 4 | 1 | 1 | MgCl | ||
| 5 | 1 | 1 | spermidine |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 30 (%(v/v)) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 4 | 1 | reservoir | ammonium acetate | 0.2 (M) |
