1KGZ

Crystal Structure Analysis of the Anthranilate Phosphoribosyltransferase from Erwinia carotovora (current name, Pectobacterium carotovorum)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	200
Detector technology	AREA DETECTOR
Collection date	2000-08-07
Spacegroup name	P 21 21 2
Unit cell lengths	77.378, 81.686, 101.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.440 *
Rwork	0.214
R-free	0.26430 *
Structure solution method	MIR
RMSD bond length	0.006 *
RMSD bond angle	1.200 *
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.067 *	0.398 *
Number of reflections	20457 *
<I/σ(I)>	37.6	7.1
Completeness [%]	98.7 *	98.6
Redundancy	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG 3000, MnCl2, glutamine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)	pH6.5
10	1	reservoir	PRPP	1.25 (mM)
11	1	reservoir	anthranilate	0.0012 (mM)
12	1	reservoir	dithiothreitol	1 (mM)
13	1	reservoir	EDTA	1 (mM)
2	1	drop	glycerol	10 (%)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop	protein	20 (mg/ml)
6	1	reservoir	PEG3000	25 (%)
7	1	reservoir	HEPES	0.2 (M)	pH6.5
8	1	reservoir	glutamine	4.5 (mM)
9	1	reservoir		10.0 (mM)