1KGI
Rat transthyretin (also called prealbumin) complex with 3,3',5,5'-tetraiodothyroacetic acid (t4ac)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS |
| Synchrotron site | CHESS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-01 |
| Detector | HAMLIN/UCSD |
| Wavelength(s) | 0.9109 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.510, 81.510, 160.238 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.204 |
| Rwork | 0.207 |
| R-free | 0.24000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | TETRAMER OF RAT TTR PDB ENTRY 1GKE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.500 * |
| Data reduction software | HAMLIN/UCSD |
| Data scaling software | UCSD-system |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 * | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.351 |
| Number of reflections | 35710 | |
| <I/σ(I)> | 24.7 | 2.3 |
| Completeness [%] | 70.2 | 28 |
| Redundancy | 4.31 | 5.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | ammonium sulphate, acetate buffer,, pH 5.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
