1KG5
Crystal structure of the K142Q mutant of E.coli MutY (core fragment)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.490, 50.120, 70.380 |
Unit cell angles | 90.00, 122.68, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.350 |
Rwork | 0.128 |
R-free | 0.16900 |
Structure solution method | Difference fourier |
Starting model (for MR) | 1kg2 |
RMSD bond length | 0.016 |
RMSD bond angle | 0.031 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 49330 | |
<I/σ(I)> | 13.5 | |
Completeness [%] | 91.7 | 55.4 |
Redundancy | 4.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | Lithium sulfate, HEPES, Sodium chloride, Magnesium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |