1KG4
Crystal structure of the K142A mutant of E. coli MutY (core fragment)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.030, 50.060, 69.880 |
Unit cell angles | 90.00, 122.60, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
Rwork | 0.149 |
R-free | 0.19700 |
Structure solution method | difference fourier |
Starting model (for MR) | 1kg2 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.024 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 30985 | |
<I/σ(I)> | 17.7 | |
Completeness [%] | 96.7 | 70.9 |
Redundancy | 4.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | Lithium sulfate, HEPES, Sodium chloride, Magnesium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |