1KFV
Crystal Structure of Lactococcus lactis Formamido-pyrimidine DNA Glycosylase (alias Fpg or MutM) Non Covalently Bound to an AP Site Containing DNA.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.880, 62.030, 80.970 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 * |
| Rwork | 0.251 |
| R-free | 0.28500 |
| Structure solution method | Molecular replacement combined with MAD |
| Starting model (for MR) | 1ee8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SnB |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.670 |
| High resolution limit [Å] | 2.500 * | 2.540 |
| Rmerge | 0.087 | 0.288 |
| Number of reflections | 21897 | |
| <I/σ(I)> | 6.1 | 1.4 |
| Completeness [%] | 98.7 | 94.3 |
| Redundancy | 3.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 293 | used microseeding * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4K | ||
| 2 | 1 | 1 | LiSO4 | ||
| 3 | 1 | 1 | TCEP | ||
| 4 | 1 | 1 | Tris-HCl | ||
| 5 | 1 | 1 | spermidine |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES-NaOH | 10 (mM) | pH7.6 |
| 10 | 1 | reservoir | spermidine | 2 (mM) | |
| 11 | 1 | reservoir | PEG4000 | 28 (%) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | glycerol | 5 (%) | |
| 4 | 1 | drop | TCEP | 1 (mM) | |
| 5 | 1 | drop | PMSF | 0.1 (mM) | |
| 6 | 1 | drop | protein | 6-10 (mg/ml) | |
| 7 | 1 | reservoir | 0.2 (M) | ||
| 8 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.7 |
| 9 | 1 | reservoir | TCEP | 1 (mM) |
