Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-10-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.880, 169.840, 64.440 |
| Unit cell angles | 90.00, 95.12, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.206 * |
| Rwork | 0.221 |
| R-free | 0.26600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.179 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.045 |
| Total number of observations | 400680 * |
| Number of reflections | 47236 |
| Completeness [%] | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 293 | Masumoto, H., (2000) Acta Crystallogr., Sect.D, 56, 73. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | DTE | 1 (mM) | |
| 6 | 1 | drop | guanidium chloride | 1 (M) | |
| 7 | 1 | reservoir | PEG10000 | 15 (%) | |
| 8 | 1 | reservoir | 2-propanol | 2.2 (%) | |
| 9 | 1 | reservoir | HEPES-NaOH | 100 (mM) | pH7.7 |
