1KEA
STRUCTURE OF A THERMOSTABLE THYMINE-DNA GLYCOSYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 1998-07-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 68.215, 68.215, 98.944 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 * | |
| Total number of observations | 209829 * | |
| Number of reflections | 16125 | |
| Completeness [%] | 99.0 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 298 | ammonium sulfate, zinc acetate, hepes, pH 8.0, EVAPORATION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 60 (%sat) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) | pH8.0 |
| 6 | 1 | reservoir | zinc acetate | 10 (mM) | |
| 7 | 1 | reservoir | dithiothreitol | 2 (mM) |
