1KDJ
OXIDIZED FORM OF PLASTOCYANIN FROM DRYOPTERIS CRASSIRHIZOMA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-02-20 |
| Detector | WEISSENBERG |
| Spacegroup name | P 61 |
| Unit cell lengths | 73.118, 73.118, 31.095 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.700 |
| R-factor | 0.241 * |
| Rwork | 0.234 |
| R-free | 0.28800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | POPLAR PLASTOCYANIN |
| RMSD bond length | 0.013 |
| RMSD bond angle | 27.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.089 * | 0.148 |
| Total number of observations | 69003 * | |
| Number of reflections | 10220 * | |
| <I/σ(I)> | 9.1 | 2.45 |
| Completeness [%] | 96.0 * | 83.3 |
| Redundancy | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | 20 * | pH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 0.1 (M) | |
| 3 | 1 | drop | ammonium sulfate | 32 (%sat) | |
| 4 | 1 | reservoir | sodium azide | 1 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 64-65 (%sat) |
