1KD8

X-RAY STRUCTURE OF THE COILED COIL GCN4 ACID BASE HETERODIMER ACID-d12Ia16V BASE-d12La16L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	86.044, 86.044, 78.384
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.900
R-factor	0.232 *
Rwork	0.232
R-free	0.27600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	calculated structure
RMSD bond length	0.018 *
RMSD bond angle	0.990 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.060	0.413
Number of reflections	25471
Completeness [%]	98.9	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	PEG 4000, Na Hepes, 2-propanol, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	18-20 (%)
3	1	reservoir	sodium HEPES	0.1 (M)	pH7.7
4	1	reservoir	2-propanol	10 (%)