1KCW
X-RAY CRYSTAL STRUCTURE OF HUMAN CERULOPLASMIN AT 3.0 ANGSTROMS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 276 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87, 0.882 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 213.920, 213.920, 85.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 3.000 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.28600 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.940 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.640 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.072 | 0.276 |
| Number of reflections | 44533 | |
| <I/σ(I)> | 7.4 | 2.7 |
| Completeness [%] | 98.5 | 93.6 |
| Redundancy | 7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.45 * | 277 * | pH 5.65 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | hCP | 28-30 (mg/ml) | |
| 2 | 1 | drop | PEG20000 | 3 (%(w/v)) | |
| 3 | 1 | drop | 250 (mM) | ||
| 4 | 1 | drop | sodium acetate | 100 (mM) |
