1KCT
ALPHA1-ANTITRYPSIN
Experimental procedure
| Temperature [K] | 295 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-11-10 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.870, 39.980, 92.420 |
| Unit cell angles | 90.00, 102.82, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.460 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 26.420 * |
| Data reduction software | MADNES |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.600 | |
| High resolution limit [Å] | 3.460 | |
| Rmerge | 0.106 | |
| Number of reflections | 4380 | |
| Completeness [%] | 82.6 | 63 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.9 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | inhibitor | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 28.5 (%(w/v)) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | lithium sulfate | 200 (mM) |
