1KCK

Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant N193G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1995-01-01
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	120.686, 111.162, 65.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.640 - 2.430
R-factor	0.153 *
Rwork	0.159
R-free	0.19900 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2cxg
RMSD bond length	0.006 *
RMSD bond angle	1.250 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	2.430
Number of reflections	31710
Completeness [%]	92.1 *	70.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.55 *	295	Lawson, C.L., (1994) J.Mol.Biol., 236, 590. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	60 (%(v/v))
2	1	reservoir	HEPES	100 (mM)	pH7.55
3	1	drop	alpha-cyclodextrin	10-20 (mg/ml)