1KCG

NKG2D in complex with ULBP3

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9639, 0.9792, 0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	62.050, 62.050, 237.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.000 - 2.600
R-factor	0.22 *
Rwork	0.220
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.890
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	2.590
High resolution limit [Å]	2.600	2.600 *
Rmerge	0.083 *	0.400 *
Number of reflections	26678 *	2683 *
Completeness [%]	99.7 *	99.9 *
Redundancy	5.4 *	5.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	297	PEG 8000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8-15 (mg/ml)
2	1	reservoir	PEG3350-8000	10 (%)
3	1	reservoir	MES	50 (mM)	pH6.0