1KBG

MHC Class I H-2KB Presented Glycopeptide RGY8-6H-GAL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	133
Detector technology	IMAGE PLATE
Collection date	1996-02-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	135.870, 86.840, 44.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 - 2.200
R-factor	0.224
Rwork	0.224
R-free	0.27900
Structure solution method	MR
Starting model (for MR)	2vaa
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.078 *	0.384 *
Number of reflections	28511	1734 *
<I/σ(I)>	16.2	1.9
Completeness [%]	89.3	55
Redundancy	5.4	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.4		2.3M Na/K PHOSPHATE, 2% MPD, pH 6.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	18 (mg/ml)
2	1	1	sodium potassium phosphate	2.3 (M)
3	1	1	MPD	2 (%)