1KAP
THREE-DIMENSIONAL STRUCTURE OF THE ALKALINE PROTEASE OF PSEUDOMONAS AERUGINOSA: A TWO-DOMAIN PROTEIN WITH A CALCIUM BINDING PARALLEL BETA ROLL MOTIF
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Collection date | 1993-02-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 65 |
| Unit cell lengths | 106.900, 106.900, 97.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.640 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.320 * |
| Data reduction software | MOSFLM (V. 5.3) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.640 |
| Rmerge | 0.050 |
| Number of reflections | 75163 |
| Completeness [%] | 98.2 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 18 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 30-40 (mg/ml) | |
| 2 | 1 | 1 | PEG8000 | 3-6 (%) |
