1KAF

DNA Binding Domain Of The Phage T4 Transcription Factor MotA (AA105-211)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795, 0.9789, 0.9636
Spacegroup name	P 1
Unit cell lengths	31.284, 54.733, 91.445
Unit cell angles	94.77, 91.10, 95.61

Refinement procedure

Resolution	30.000 - 1.600
R-factor	0.226 *
Rwork	0.226
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.007 *
RMSD bond angle	1.310 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.100 *
Total number of observations	638591 *
Number of reflections	87713 *
Completeness [%]	92.0	83 *
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5 *	298	from 19% PEG 8000, 35 mM potassium phosphate pH 5.0, 3% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	19 (%)
2	1	reservoir	potassium phosphate	35 (mM)
3	1	reservoir	glycerol	3 (%)