1KA9
Imidazole Glycerol Phosphate Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Collection date | 2001-09-21 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 82.259, 82.259, 156.183 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
Rwork | 0.203 |
R-free | 0.25600 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 25.100 * |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.300 * | 2.300 * |
Rmerge | 0.063 | 0.261 * |
Total number of observations | 88635 * | |
Number of reflections | 23969 * | |
Completeness [%] | 98.8 | 99.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | hepes, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | sodium HEPES | 10 (mM) | |
3 | 1 | reservoir | 4.4 (M) | ||
4 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |