1K9S
PURINE NUCLEOSIDE PHOSPHORYLASE FROM E. COLI IN COMPLEX WITH FORMYCIN A DERIVATIVE AND PHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90950 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 178.779, 178.779, 167.943 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.000 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.18300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.106 | 0.412 * |
| Total number of observations | 622701 * | |
| Number of reflections | 178749 | |
| Completeness [%] | 98.2 | 97.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 295 | Cook, W.J., (1985) J. Biol. Chem., 260, 12968. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 15 (%sat) | |
| 3 | 1 | drop | citrate | 0.025 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 30 (%sat) | |
| 5 | 1 | reservoir | citrate | 0.05 (M) |
