1K9B
Crystal structure of the bifunctional soybean Bowman-Birk inhibitor at 0.28 nm resolution. Structural peculiarities in a folded protein conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-04-05 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 86.100, 86.100, 86.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| Rwork | 0.221 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pi2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | ROTAVATA |
| Data scaling software | WEIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.900 | 3.000 |
| High resolution limit [Å] | 2.500 | 2.800 |
| Rmerge | 0.058 | 0.300 |
| Number of reflections | 26756 | |
| Completeness [%] | 96.3 | 70 |
| Redundancy | 6.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 289 | PEG 4000, ammonium sulfate, pH 7.6, VAPOR DIFFUSION, SITTING DROP at 289K |
