1K8K

Crystal Structure of Arp2/3 Complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.08
Spacegroup name	P 21 21 21
Unit cell lengths	111.710, 130.400, 204.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.010 *
R-factor	0.216 *
Rwork	0.216
R-free	0.24900 *
Structure solution method	MAD
RMSD bond length	0.011 *
RMSD bond angle	1.353 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.010 *
Rmerge	0.064 *
Total number of observations	852046 *
Number of reflections	183319 *
<I/σ(I)>	8.6
Completeness [%]	92.8 *
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	4 *	PEG 8000, KSCN, Hepes at pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris	20 (mM)	pH8.0
3	1	drop		100 (mM)
4	1	drop	EGTA	1 (mM)
5	1	drop		1 (mM)
6	1	drop	dithiothreitol	1 (mM)
7	1	reservoir	PEG8000	6 (%)
8	1	reservoir	KSCN	0.1 (M)
9	1	reservoir	HEPES	0.1 (M)	pH7.5