1K8G
Crystal Structure of the N-terminal domain of Oxytricha nova telomere end binding protein alpha subunit both uncomplexed and complexed with telomeric ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 121.182, 121.182, 137.169 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | residues 37-320 of Oxytricha nova telomere end binding protein alpha subunit PDB accession 1OTC |
RMSD bond length | 0.009 |
RMSD bond angle | 1.557 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.103 * | 0.710 * |
Total number of observations | 574134 * | |
Number of reflections | 35994 | |
<I/σ(I)> | 20.3 | 2.1 |
Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG4000, MES, Ammonium sulfate, DTT, Sodium Azide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG4000 | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | NH4SO4 | ||
4 | 1 | 1 | DTT | ||
5 | 1 | 1 | Sodium Azide |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 5 (mM) | pH7.5 |
10 | 1 | reservoir | 0.02 (%) | ||
11 | 1 | reservoir | 50 (mM) | ||
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | dithiothreitol | 2 (mM) | |
4 | 1 | drop | EDTA | 0.1 (mM) | |
5 | 1 | drop | 0.02 (%) | ||
6 | 1 | reservoir | PEG4000 | 22 (%(w/v)) | |
7 | 1 | reservoir | MES | 50 (mM) | pH6.6 |
8 | 1 | reservoir | ammonium sulfate | 150 (mM) | |
9 | 1 | reservoir | dithiothreitol | 2 (mM) |