1K7T

Crystal Structure Analysis of crosslinked-WGA3/GlcNAcbeta1,6Gal complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Detector technology	AREA DETECTOR
Collection date	2000-12-29
Detector	ENRAF-NONIUS FAST
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	45.150, 93.170, 45.100
Unit cell angles	90.00, 110.81, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.242 *
Rwork	0.242
R-free	0.32600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Native WGA3
RMSD bond length	0.012
RMSD bond angle	24.200 *
Data reduction software	MADNESS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.450
High resolution limit [Å]	2.400	2.400
Rmerge	0.116	0.249
Total number of observations	32097 *
Number of reflections	13176 *
Completeness [%]	96.2
Redundancy	2.44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.9	298	Harata, K., (1995) Acta Crystallogr., Sect.D, 51, 1013. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	WGA3	2.5 (mg)
2	1	2	acetate	10 (mM)	pH4.9
3	1	2		6 (mM)
4	1	2	ethanol	4 (%(v/v))